ADME & in vitro Pharmacology Services

• Aqueous solubility (PDF)
• Chemical stability (specific assay conditions) (PDF)
• Lipophilicity by CHI (PDF)

Metabolic stability determination in vitro (various species)

• Microsomes (Phase I and/or glucuronidation, various species) (PDF)
• Hepatocytes (various species) (PDF)

P450 profiling (PDF)

• Rapid screen (recombinantly expressed single P450 enzymes)
• Probe substrate approach with microsomes (meets FDA guidelines)

P450 inhibition

• Evaluation of direct and metabolism- dependent inhibition of Cytochrome P450 enzymes (PDF)
• Rapid screen (recombinantly expressed single P450 enzymes) (PDF)
• Probe substrate approach with liver microsomes (meets FDA guidelines) (PDF)

P450 induction (PDF)

Metabolism assay in HepaRG cell line (PDF)

Plasma, serum or blood stability (PDF)

Permeability screen in vitro on artificial membrane

• PAMPA (prediction of intestinal absorption) (PDF)
• BBB (prediction of CNS penetration) (PDF)

Permeability screen in vitro on Caco2 cell monolayers (PDF)

• Pgp affinity (bidirectional transport in Caco2) (PDF)
• Pgp inhibition (ATPase assay) (PDF)

Characterization of transporter interactions (PDF)

Plasma or serum protein binding (PDF)

Determination of Kd for HSA and AGP (PDF)

In vitro cytotoxicity (PDF)

Histamine release ex vivo in rat mastocytes (PDF)